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Antioxidizing capacities of ruthenium red, 2‐APB, and BAPTA‐AM interfere with the analysis of a potential Ca 2+ ‐dependency of BH4‐ or H 2 O 2 ‐induced ERK1/2 signaling in rat dorsal root ganglion neurons. (A–D) Relative pERK1/2 intensity in ex vivo cultured rat DRG neurons (UCHL1 + ) exposed to BH4 (100 μM) or H 2 O 2 (25 μM) after treatment with the indicated compounds. Statistical significance tested by one‐way ANOVA with Bonferroni multiple comparison testing (A, B, D) or by unpaired, two‐sided t‐ test (C), in comparison to 0 μM compound (* p < 0.05). Data presented as individual data points representing biological replicates and mean ± SD. Experiments have been performed with independent DRG preparations from n = 4–5 animals depicted by individual data points. (E) Colorimetric analysis of total antioxidant capacity of the indicated reagents in PBS using the Total Antioxidant Capacity Colorimetric Assay Kit, following manufacturer's instructions with independent Trolox standard curves (0–1.43 mM) for each replicate. Data presented as individual data points representing biological replicates and mean ± SD. (A–E) Data presented as individual data points representing biological replicates and mean ± SD (A: BH4, F (2, 9) = 14.08, p = 0.0007; H 2 O 2 , F (2, 9) = 6.612, p = 0.0116; B: BH4, F (2, 9) = 18.37, p = 0.0007; H 2 O 2 , F (2, 9) = 50.90, p < 0.0001; D: BH4, F (2, 9) = 12.07, p = 0.0013; H 2 O 2 , F (2, 12) = 5.161, p = 0.0241).

Journal: Journal of Neurochemistry

Article Title: BH4 Oxidation‐Derived H 2 O 2 Activates ERK1 /2 Signaling via B‐Raf in Rat Dorsal Root Ganglion Neurons

doi: 10.1111/jnc.70271

Figure Lengend Snippet: Antioxidizing capacities of ruthenium red, 2‐APB, and BAPTA‐AM interfere with the analysis of a potential Ca 2+ ‐dependency of BH4‐ or H 2 O 2 ‐induced ERK1/2 signaling in rat dorsal root ganglion neurons. (A–D) Relative pERK1/2 intensity in ex vivo cultured rat DRG neurons (UCHL1 + ) exposed to BH4 (100 μM) or H 2 O 2 (25 μM) after treatment with the indicated compounds. Statistical significance tested by one‐way ANOVA with Bonferroni multiple comparison testing (A, B, D) or by unpaired, two‐sided t‐ test (C), in comparison to 0 μM compound (* p < 0.05). Data presented as individual data points representing biological replicates and mean ± SD. Experiments have been performed with independent DRG preparations from n = 4–5 animals depicted by individual data points. (E) Colorimetric analysis of total antioxidant capacity of the indicated reagents in PBS using the Total Antioxidant Capacity Colorimetric Assay Kit, following manufacturer's instructions with independent Trolox standard curves (0–1.43 mM) for each replicate. Data presented as individual data points representing biological replicates and mean ± SD. (A–E) Data presented as individual data points representing biological replicates and mean ± SD (A: BH4, F (2, 9) = 14.08, p = 0.0007; H 2 O 2 , F (2, 9) = 6.612, p = 0.0116; B: BH4, F (2, 9) = 18.37, p = 0.0007; H 2 O 2 , F (2, 9) = 50.90, p < 0.0001; D: BH4, F (2, 9) = 12.07, p = 0.0013; H 2 O 2 , F (2, 12) = 5.161, p = 0.0241).

Article Snippet: Colorimetric analysis of total antioxidant capacity has been performed using the Total Antioxidant Capacity Colorimetric Assay Kit (Elabscience, cat. no. E‐BC‐K219‐M) following the manufacturer's instructions with independent Trolox standard curves (0–1.43 mM Trolox) for each experimental replicate and 1 mM ascorbic acid as a positive control.

Techniques: Ex Vivo, Cell Culture, Comparison, Colorimetric Assay

ABTS assay of ripe and unripe Annurca apple samples. ( A ) Flesh samples. ( B ) Peel samples. ( C ) Core samples. ( D ) Differences between samples of the different components. ( E ) Standards for positive control. Statistical analysis is performed using one-way ANOVA followed by Tukey’s test. *** ( p < 0.001), ** ( p < 0.01), * ( p < 0.05), and ns ( p > 0.05).

Journal: Antioxidants

Article Title: Antioxidant Activity of Annurca Apple By-Products at Different Ripening Stages: A Sustainable Valorization Approach

doi: 10.3390/antiox14080941

Figure Lengend Snippet: ABTS assay of ripe and unripe Annurca apple samples. ( A ) Flesh samples. ( B ) Peel samples. ( C ) Core samples. ( D ) Differences between samples of the different components. ( E ) Standards for positive control. Statistical analysis is performed using one-way ANOVA followed by Tukey’s test. *** ( p < 0.001), ** ( p < 0.01), * ( p < 0.05), and ns ( p > 0.05).

Article Snippet: ABTS assay kit (E-BC-K271-M) was from ElabScience (Houston, TX, USA).

Techniques: ABTS Assay, Positive Control